Detection of high mass molecular ions by laser desorption time-of-flight mass spectrometry.

Desorption Ionization-Time of Flight Mass Spectrometry

Advances in the Identification of Clinical Yeast Isolates Using Matrix-Assisted Laser 1 Desorption Ionization-Time of Flight Mass Spectrometry 2 3 Blake W. Buchan and Nathan A. Ledeboer 4 Department of Pathology, Medical College of Wisconsin, and Dynacare Laboratories, 9200 W. 5 Wisconsin Ave., Milwaukee, WI 53226. 6 7 Running Title: Identification of Yeast Using MALDI-TOF 8 9 Correspondence: N...

matrix-assisted laser desorption/ionization time of flight mass spectrometry: a new guide to infectious disease

Isoelectric Focusing, Matrix-assisted Laser Desorption/ionization Time-of- Flight Mass Spectrometry, Electrospray Ionization Mass Spectrometry

LEVEL: INTERMEDIATE D iscovery, development, and commercialization of novel biologics frequently involve collaboration between two or more companies. In the context of these business relationships, transfer of technology from one institution to another is a crucial step that needs to be executed f lawlessly and rapidly. Follow-up activities usually include the development of productive, reliabl...

Bacteriocin detection from whole bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Class I bacteriocins (lantibiotics) and class II bacteriocins are antimicrobial peptides secreted by gram-positive bacteria. Using two lantibiotics, lacticin 481 and nisin, and the class II bacteriocin coagulin, we showed that bacteriocins can be detected without any purification from whole producer bacteria grown on plates by matrix-assisted laser desorption ionization-time of flight mass spec...

Analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

MALDI-MS is one of the most useful techniques available for determining biomolecule mass. It offers high mass accuracy, good sensitivity, simplicity, and speed. Because singly charged ions of oligonucleotides are typically observed, MALDI-MS spectra are easy to interpret. This unit presents protocols for sample preparation and purification, matrix preparation, and matrix/analyte sample preparat...